rok [2017-8-11 16:0:3]

repressor of comK, nucleoid associated protein that serves to help repress expression of A T-rich genes, many of which appear to have been acquired by horizontal gene transfer

locus: BSU14240

pI: 9.70

mw: 21.69 kDa

protein length: 191 aa

gene length: 573 bp

function: regulation of genetic competence

product: transcriptional repressor

essential: no

synonyms: ykuW

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.7|Genetic competence]
• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.5|Transcription factors/ other]
• [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.3|Genetic competence]

Gene

Coordinates: 1,493,787 → 1,494,362

Phenotypes of a mutant

• higher excision rate of the conjugative transposon ICEBs1 [Pubmed|21085634]

The protein

Catalyzed reaction/ biological activity

• binds to extended regions of the ''B. subtilis'' genome that are characterized by a high A+T content and are known or believed to have been acquired by horizontal gene transfer [Pubmed|21085634]

Effectors of protein activity

• activity is modulated by interaction with [protein|6740108089F13116F200C15F35C2E7561E990FEB|DnaA] [Pubmed|27902860]

Expression and Regulation

Operons

• genes: [gene|1508770D5BCA5739D13E3ABD11CC5C103C07FF25|rok]
  description: [Pubmed|12354229]

  regulatory mechanism

  • [protein|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A]: repression, [Pubmed|14651647], in [regulon|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A regulon]
  • [protein|5A6FBAE6553343092862CB79E150F934978C32A9|SinR]: repression, [Pubmed|11849533], in [regulon|5A6FBAE6553343092862CB79E150F934978C32A9|SinR regulon]
  • [protein|1508770D5BCA5739D13E3ABD11CC5C103C07FF25|Rok]: repression, [Pubmed|11849533], in [regulon|1508770D5BCA5739D13E3ABD11CC5C103C07FF25|Rok regulon]
  • [protein|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB]: repression, [Pubmed|11849533], in [regulon|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB regulon]

  regulation

  • repressed by [protein|search|Rok] [Pubmed|11849533]

Biological materials

Mutant

• MGNA-B345 (ykuW::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1344 NBRP B. subtilis, Japan]

References

Reviews

• 19995980

Original publications

• 21085634,12354229,14651647,11849533,21097620,15743949,27375604,27902860